ABSTRACT
Objective To investigate the molecular characteristics of Japanese encephalitis virus (JEV) living in vector mosquitoes,from Zhejiang province.Methods A total of 13620 mosquitoes were collected from the monitoring stations located in Cixi city and Xianju county in Zhejiang province,in July and August,2009-2010.Nucleic acid of JEV from the mosquitoes was monitored by using real-time RT-PCR.The virus strains were isolated with BHK-21 cell line,with E genes of the isolated viruses amplified,sequenced and their phylogeny and homology analyzed.Results The positive rates of JEV for those mosquitoes collected in the stations of Cixi and Xianju were 17.0％ (27/159) and 3.4％ ( 1/29 ),respectively.Twenty-two JEV strains were isolated,accounted for 15.4％ among the 143 batches of mosquitoes collected in 2010.All E genes in the 6 sequenced virus isolates contained 1500 nucleotides encoding 500 amino acids,in which no inserts and deletions were identified.The identity rates of nucleotide and amino acid in E gene were 99.2％-99.8％ and 100.0％ among the 6 JEV strains isolated from Zhejiang,99.1％-99.3％ and 99.2％-99.8％ between the Zhejiang strains in 2009-2010 and the Zhejiang strains in 2007-2008,respectively,87.6％-88.0％ and 97.8％ between the 6 Zhejiang strains and the vaccine strain SA 14-14-2 of JEV,respectively.The phylogeny tree of E gene indicated that the JEV isolates in Zhejiang during 2009-2010 was located in the branch of the genotype Ⅰ.Conclusion Mosquitoes collected from Cixi and Xianju areas carried JEV,with the rate of JEV in Cixi higher than in Xianju.All the Zhejiang isolates in 2009-2010 were proven to be the genotype Ⅰ of JEV.
ABSTRACT
Objective To collect more data on the epidemiology of hantavirus in rodents in Cixi,Zhejiang province.Methods Rodents were captured in Cixi,where hemorrhagic fever with renal syndrome (HFRS)appeared endemic.Hantavirus antigens in the rat lungs were detected by immunofluorescence assay.Partial S segment sequences were amplified by reverse transciption-polymerase chain reaction(RTPCR)and then sequenced.The phyologenetic trees were constructed by maximum likelihood method to detect the genetic characteristics of hantavirus.Results A total of 243 rodents were trapped in the epidemic areas,and hantavirus antigens were identified in 7 out of these lung samples(2.88%).Partial S segment sequences(620-999 nt)were recovered from 6 samples and sequenced.Data from phylogenetic analysis of these S segment sequences indicated that all viruses belonged to Seoul virus(SEOV),despite the origins of sources were either from Rattus norvegicus or from R.fzabipectus.These viruses could further be divided into two distinct lineages but the viruses carried by R.norvegicus were different from those carried by R.flabipectus.Conclusion Two distinct lineages of SEOV had been cocirculating in rodents in Cixi.